FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_chip_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_chip_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26207926
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG7085362.703518012070089TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC805000.066.0695144
TATGCCG806400.066.01092546
GTATGCC808350.065.84704645
ATGCCGT819850.064.96165547
CACAGTG821550.064.419333
TGCCGTC827300.064.3932248
GTCACAC827900.064.0461629
TCTCGTA821800.063.6023441
ACGTCTG847250.063.37682715
CAGTGAT837550.062.80560335
GCCGTCT848250.062.78681649
CGTCTGA855700.062.74695216
ACAGTGA842750.062.6718834
GCACACG858350.062.64734611
ATCTCGT832900.062.61388840
ACACGTC863100.062.284813
CACACGT862800.062.283312
TCGTATG858100.061.96475243
CTCGTAT850550.061.76061242
ACACAGT860950.061.43506632
Produced by FastQC (version 0.11.2)