FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_chip_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_chip_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23056952
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG7056533.060478245346566TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC790350.066.9189644
TATGCCG793700.066.6496846
GTATGCC800150.066.1118545
TCTCGTA788600.065.886341
ATGCCGT814250.065.05231547
ATCTCGT802850.064.7297640
CACTTAG819700.064.0510831
TAGGCAT820200.063.90323635
CATCTCG813000.063.8579939
CGTCTGA833750.063.77498616
TCGTATG830800.063.66495543
CTCGTAT822750.063.6213142
ACGTCTG837950.063.53767415
GCCGTCT834850.063.4391649
ACACGTC841250.063.39081213
GCATCTC822500.063.1292138
TGCCGTC843150.062.8512248
CCGTCTT843000.062.82256350
CACACGT848650.062.8100212
AGTCACT840300.062.59198828
Produced by FastQC (version 0.11.2)