FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_chip_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_chip_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27031472
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG8849773.27387646518103TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1019850.066.4132744
TATGCCG1019800.066.34601646
GTATGCC1024050.066.1274645
ATGCCGT1029200.065.79381647
TGCCGTC1033500.065.5267648
TCTCGTA1022200.064.2717841
ACGTCTG1067000.063.93703515
ACTGACC1053800.063.6899732
GCCGTCT1063800.063.6863149
AGTCACT1061650.063.55698828
CGTCTGA1074100.063.46610616
ATCTCGT1035050.063.44730440
GCACACG1079100.063.35566711
AATCTCG1036850.063.3337139
ACACGTC1084200.063.01476713
CTCGTAT1048150.062.9081142
CACACGT1087500.062.83094412
TCGTATG1078800.062.79330443
CTGACCA1072700.062.55471433
CTGAACT1087500.062.50914819
Produced by FastQC (version 0.11.2)