FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_chip_300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_chip_300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21364568
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG16098757.5352565050695155TruSeq Adapter, Index 6 (100% over 63bp)
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTAT254210.11898672605970782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1782600.067.2960644
TATGCCG1783650.067.2339346
GTATGCC1784200.067.2301445
ATGCCGT1790250.066.9939347
CACGCCA1788000.066.83526631
TGCCGTC1796400.066.7663448
TCTCGTA1788600.066.66881641
TCACGCC1792600.066.65205430
GCCAATA1791200.066.6096234
ATCTCGT1800650.066.2437440
ACGTCTG1834000.066.1810915
CGTCTGA1836800.066.0182216
CTGAACT1835150.065.8438619
ACACGTC1848200.065.8015613
CCAATAT1815450.065.7947635
GCCGTCT1824000.065.7615849
TGCTTGA1826150.065.7588958
TCTGAAC1840750.065.7447618
AGTCACG1822000.065.7417428
CACACGT1852400.065.6744112
Produced by FastQC (version 0.11.2)