FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_chip_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_chip_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24596046
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG14731555.989397645458949TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1653850.066.7586245
CGTATGC1652850.066.7573644
TATGCCG1656100.066.67068546
ATGCCGT1660000.066.5015747
TGCCGTC1667250.066.191748
ACCGATG1673550.065.56212632
TCTCGTA1664950.065.4895141
AGTCACC1681750.065.437628
GCCGTCT1690150.065.2846649
GTCACCG1690650.065.0441429
ATCTCGT1677100.065.0006640
CTCGTAT1683950.064.86928642
TCGTATG1698800.064.856143
CCGATGT1694400.064.7369133
CGATGTA1695050.064.7119634
CCGTCTT1708650.064.6250450
TATCTCG1688400.064.5308939
ACGTCTG1740450.064.2240415
TGCTTGA1719150.064.2216158
GCTTGAA1723300.064.1547859
Produced by FastQC (version 0.11.2)