FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_chip_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_chip_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24921043
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1623640.6515136625702223TruSeq Adapter, Index 1 (100% over 63bp)
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTAT294220.1180608692822367No Hit
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATC264860.10627966092751416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC202750.060.8126244
GTATGCC203850.060.47154245
TATGCCG207450.059.47227546
ATGCCGT216300.057.2304747
TCTCGTA219850.054.87454641
TGCCGTC227000.054.57819748
GTCACAT232900.052.55459229
ATCTCGT232450.051.8704840
ATCACGA237600.051.42819634
TGCTTGA253350.048.8041458
GCTTGAA255850.048.42135659
ACGTCTG261750.048.0008515
CGTCTGA264150.047.4471816
CTGAACT268250.046.70922519
GCCGTCT267000.046.4922349
TCGTATG271150.045.7264643
TCTGAAC274500.045.6581918
ACACGTC276150.045.5601913
CACACGT277750.045.31097412
CTCGTAT271050.045.0179542
Produced by FastQC (version 0.11.2)