FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37224691
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28243067.587184538348485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA565150.059.036911
CGAAAGC223400.055.3744623
GAGCGAA137100.054.9885441
GCAAAAG641350.051.3523943
CAAAAGC758750.044.520134
AGCAAAA771900.043.001012
AAGCAGG1036600.038.289317
AGCGAAA338800.036.5025631
AGCAGGG512350.035.7753948
AAAGCAG1201750.033.91756
AAAAGCA1025900.033.5520065
GCAGGGT331300.032.7416849
GCGAAAG384250.032.3482252
GCAGGTA270600.028.1325289
ACAAGGG256350.026.5498373
GAAAGCA509750.025.1323434
AGTAGAA314900.021.7304421
AACAAGG552500.021.7293762
AGCAGGC241450.021.0256678
GTAGAAA444650.020.596151
Produced by FastQC (version 0.11.5)