FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7720001
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG257410.33343259929629543No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC157700.20427458493852527No Hit
CTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATAAAA148350.19216318754362854No Hit
TACTAGGGACGGCCCTTCTCAAATTTCCTGCGCGCACAGAGGATAGGGAC126870.1643393569508605No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACG118660.15370464330250735No Hit
GTTTAATTTTGAGGATGGTTATTTTTATCCAGCTAAGGAAAGCATCGATA92750.12014247148413582No Hit
TAGCCAACCAGCCATGCACCTGGCGGTACAACTGGCACACCAGAGGTTCG92180.11940412961086405No Hit
GATAAAAGGTACCCCGGGGATAACAGGCTGATCTTGCCCAAGAGTCCATA88480.11461138411769636No Hit
AAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAACGT86760.11238340513168327No Hit
GAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATA86320.11181345701898225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80380.10411915749751846No Hit
ATATTGACCTGCTGCCAACATACAACCGACCTTATTTTCAGGGTCAATCT79180.1025647535537884No Hit
ATACTGACCTGCTGCCAACATACAACCAACCTTATTTTCAGGGTCAATCT77520.10041449476496182No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA221450.084.853741
GCAAAAG282600.066.184573
AAAAGCA289050.065.1018145
AGCAAAA290400.064.496822
CGAAAGC106150.056.2068253
CAAAAGC338700.055.5171854
AAGCAGG378100.054.7344637
GCGAAAG111550.053.1733782
AGTAGAA89100.051.2176781
GCAGGCT114650.047.6714869
GAGCGAA77450.047.3060721
AGCGAAA129000.045.6118851
AGCAGGC159750.044.7855228
AAAGCAG523200.042.5335926
GGTCGAT21300.039.511661
CCTATGC42750.034.9333573
ACAAGGC71100.034.8711053
TAGTGCG20850.034.7660527
GTCCTAT44450.034.6027831
AGTGCGC21200.034.192088
Produced by FastQC (version 0.11.5)