FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383753
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA43900.18416337598736113No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG33920.1422966221752002No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA31540.13231236625606763No Hit
GCAGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT31540.13231236625606763No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT31010.13008898153458012No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT30470.12782364615797023No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT30120.126355373228686No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA29070.12195055444083343No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT27690.11616136403394145No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA26430.11087558148851831No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG24690.1035761674972197No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG24650.10340836487673011No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT24490.10273715439477162No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA24070.10097522687963056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9200.0101.7570651
CGAAAGC28950.080.384533
GCGAAAG29650.078.258672
GAGCAAA62550.071.236691
AGCGAAA35500.064.802771
GCAAAAG92600.047.287023
AGTAGAA34650.047.123981
CAAAAGC94350.046.557764
AAGCAGG108100.046.20047
TAGAAAC42850.045.5206642
GTAGAAA43450.044.9288371
CAATTCG7150.044.6065759
AGCAAAA99550.044.3591422
GAAAGCA56200.043.0795864
AAAAGCA112800.039.135385
GCAGGGT28000.037.2783559
AAAGCAG144000.036.9979026
AGCAGGG59600.033.9320568
GCAGGTA22550.033.7516489
GTCACCG2800.033.6590083
Produced by FastQC (version 0.11.5)