FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5179828
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG228190.44053586335299166No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA133860.2584255693432292No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA113780.21965980337571053No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA100180.1934041053100605No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT87770.1694457808251548No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG83860.16189726763128043No Hit
GTACTATGTTCTTTTCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG81350.1570515468853406No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT80370.1551595921717864No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC79630.15373097330644955No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC77640.14988914689831398No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT75930.14658787897976536No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT74630.1440781431352547No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA66840.12903903372853306No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT65510.12647138090299523No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC65260.1259887393944355No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG63090.12179941110013691No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT62080.11984953940555555No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT55870.1078607243329315No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT55750.10762905640882284No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC53500.10328528283178515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA26450.0109.985011
GAGCAAA178000.074.380251
GCGAAAG51400.066.3014452
CGAAAGC53600.063.7043233
GCAGGTA49850.058.7460569
AGCAAAA225450.058.0195732
AAGCAGG272000.057.5640757
GCAGGGT116700.054.1636779
GCAAAAG247050.053.2897643
CAAAAGC252000.052.756244
AGCAGGG161350.052.6976858
GTAGAAA57400.049.796531
AGGCTCG5350.048.809074145
AAAGCAG332250.048.413136
AGCGAAA74550.045.542091
TAGAAAC62650.045.4841162
AGTAGAA42800.044.239631
AAAAGCA304400.043.936215
CAATTCG8200.038.8956769
AGCAGGT112600.036.823088
Produced by FastQC (version 0.11.5)