FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3652747
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTATAGTGATTCGTTTCCCAAGATAAAAAGCCGCTATGTAAGCATCT68710.18810500699884225No Hit
GTATCAGTACACGGCACAGGCAGAAAACAGCCTGCAAATCACCCTACCGA64630.17693533113571785No Hit
CTATAGAGAAAGCTAAACAAATAGAAAAATAGAACCTATAAATAAAAACT46670.12776685601274876No Hit
GTATATGTGTATTATTACGGCAATGCTAAACGACACAAAGAGGCTCTTAC46390.12700030962998532No Hit
CACATATACTTTGCCGTCTTTTTCTGTTGAAATAGGCGAGAGCCCTTTGA43870.12010139218511437No Hit
GTATACTTATCGAGACGGCCTTTCTTACTAGTAACGAAGAGCGAAAGTTC41820.11448917759702493No Hit
ATCTTACAAAGTGCAAATTTCGTCTAGCCCCAAACGTTTGGAAGCTAAGC37600.10293622854251883No Hit
CTATAATACTCATTCTTGCAGTCGTGAATACACAGGCACAGGAACGACAA37410.10241607206850079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG82200.046.4036872
CGAAAGC83200.046.2659073
AGCGAAA91150.041.9521561
GAAAGCA108600.036.308264
AAGCAGG129700.035.039996
GAGCAAA73450.029.1427541
AGCAGGG61450.028.78097
AAAGCAG201000.026.1447375
AGCAGGC46550.025.225037
AGCAAAA90750.023.0135942
TATAGTG64250.021.4349675
CAAAAGC101450.020.5767524
GCAGGGT22700.020.4357478
CTATAGT65100.019.818714
GCAGGGC18900.019.5589168
TCTATAG70900.019.5288663
GCAGGGG26500.019.4199838
GCAGGGA43450.019.1842338
TATTATA79300.019.1122422
GTACACG52600.018.6030627
Produced by FastQC (version 0.11.5)