FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792916
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG26580.14825011322337464No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC25240.14077625499465676No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA24650.13748552637156453No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATAACACTGAAATTCGCTT23650.13190802023073026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA13000.088.1850741
GAGCAAA66200.072.3381351
GCAAAAG70550.066.584263
AGCGAAA22250.064.2417761
CAAAAGC73950.063.815224
CGAAAGC22500.062.82693
GCGAAAG23250.061.1307832
AGCAAAA78200.059.9962842
AAGCAGG108700.050.6833767
AAAAGCA95500.049.7186555
AAAGCAG122500.046.926546
TACGGGC1002.7378137E-643.494344
TCGCTCC4500.041.8834345
AGTAGAA23800.038.717571
AGCAGGG59850.038.5152178
GCTTTCG5600.036.2786671
CGCTCCC5000.036.2452776
CGCGGAT800.002038626236.244278
TAGAAAC31150.035.6172372
AGCAGGT43900.035.1709758
Produced by FastQC (version 0.11.5)