FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4637640
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG214820.4632097359864069No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC144810.3122493337128367No Hit
TACTAGGGACGGCCCTTCTCAAATTTCCTGCGCGCACAGAGGATAGGGAC111860.24120026565235766No Hit
CTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATAAAA96490.20805840901838005No Hit
TAGCCAACCAGCCATGCACCTGGCGGTACAACTGGCACACCAGAGGTTCG74930.16156924642706205No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACG63630.13720340518022098No Hit
GATAAAAGGTACCCCGGGGATAACAGGCTGATCTTGCCCAAGAGTCCATA62300.13433556722815915No Hit
GAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG58920.1270473775454757No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA52480.11316100430391321No Hit
GCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAA50800.10953847215394036No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49510.10675688496735408No Hit
AAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAACGT49170.10602375346081196No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG48090.10369498279297229No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC47220.10181902864387922No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA178350.095.753491
AGCAAAA199900.084.004662
GCAAAAG211050.079.585513
AAAAGCA232900.072.270775
CAAAAGC233350.072.193524
GAGCGAA58900.068.852191
AAGCAGG313650.064.111487
AGCAGGC110350.063.7833678
GCAGGCT89350.060.034069
AAAGCAG365850.056.7478266
AGTAGAA50800.053.9820481
GCGAAAG75800.052.7914053
AGCGAAA77650.051.4791531
CGAAAGC82500.048.5919763
ATTTCCG10900.046.582973145
AGCAGGG111800.044.4129188
AGCAGGA138150.038.302988
CGTAGGA8150.038.270706145
CGTCGAC2500.037.6944967
GTAGAAA90200.036.514941
Produced by FastQC (version 0.11.5)