FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6107061
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62620.10253704687082706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG60600.081.246432
AGCGAAA62100.079.324231
CGAAAGC66750.073.850083
GAGCGAA26100.061.7078171
GAGCAAA143000.054.0815241
GCAAAAG178850.042.4376223
CAAAAGC182800.041.3613134
AGCAAAA207650.036.7708442
AAAAGCA252450.030.4657575
AAAGCAG302050.030.4067336
AAGCAGG292250.029.31787
GAAAGCA185150.027.4069394
TGACGCG3555.456968E-1226.56179145
ACGACGC8500.021.3194148
TACGACG9250.019.5914557
CGACGAA4954.7293724E-1019.049362145
TATACCG4253.2856406E-818.7618545
AGCAGGG202850.018.7245128
GTATATA63950.016.6764831
ATACGAC11750.016.0401156
Produced by FastQC (version 0.11.5)