FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7671657
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG165690.21597680918216236No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG143520.18707822834102203No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA107250.13980030650483982No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA102900.13413008428296522No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC96810.12619177317234073No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC92720.12086046078441724No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG85550.11151437036353423No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85520.11147526538269374No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG84860.11061495580420241No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC84470.11010659105327571No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT80210.10455368377392264No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT77790.10139921531945445No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG91850.083.6069262
GAGCGAA36150.079.6787951
CGAAAGC99900.076.845283
AGCGAAA100400.076.094831
GAGCAAA233200.063.1268421
CAAAAGC282050.051.503044
GCAAAAG313550.046.3778763
AAGCAGG377800.043.745297
AGCAAAA347800.042.095162
AAAAGCA356800.041.119415
AAAGCAG433900.040.0776146
GAAAGCA205850.038.3476074
AGTAGAA103100.035.960261
TAGAAAC121300.035.689382
GGTCGTT36950.035.50777
CAATTCG67100.035.0012669
GTAGAAA131750.034.2531361
AAGGTCG54150.033.1981939
AGCAGGT165500.032.279378
AGGTCGT47000.029.1491036
Produced by FastQC (version 0.11.5)