FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6999373
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77180.11026701963161556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG81550.093.186772
CGAAAGC87750.087.075423
AGCGAAA90850.083.214451
GAGCGAA25950.069.058241
GAGCAAA134950.048.0640751
GAAAGCA190100.041.6785134
CTCGATA30000.033.1027579
AAGCAGG321900.032.967426
AAAGCAG346350.032.837935
CAAAAGC201900.032.241424
GCAAAAG205150.031.8033663
AGCAAAA219850.029.3219072
CCTCGAT36800.027.182928
GGCCTCG39050.025.987946
AGCAGGG217500.025.5290347
AAAAGCA273050.024.317635
CCGACGC15650.024.100897145
GCCTCGA44200.023.1239347
TATACGC4205.2750693E-1122.4366999
GACGACG4952.1827873E-1120.514788145
Produced by FastQC (version 0.11.5)