FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6157095
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC102350.080.389533
AGCGAAA108950.074.648171
GCGAAAG148100.055.2777372
GAGCAAA148950.043.3988231
GCAAAAG163150.039.2342223
GAAAGCA226550.037.884494
GAGCGAA65800.035.3932081
AAGCAGG296350.035.1488846
AGCAAAA188850.033.750742
CAAAAGC197550.032.767874
AAAGCAG412200.029.3854055
AGCAGGG173650.026.2979537
GTATAGA55450.023.2894521
AAAAGCA292850.022.6732355
AGCAGGC105150.020.2672217
TCTATAC53000.018.8753363
GTGCGTT19950.018.1670029
GCAGGGG79150.017.8581458
GGTCGAT6950.017.7304737
GTGACTG57550.017.507569
Produced by FastQC (version 0.11.5)