FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2779533
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA74330.2674190232675777No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG71870.25856861566313477No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC65300.23493155145126896No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT63470.22834771164796389No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC49200.1770081520888581No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA41480.14923370220824864No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT39240.14117479447086975No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC38600.1388722494030472No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC37100.13347565940033812No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC36420.13102920526577666No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA36020.12959011459838757No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA35410.12739550133061922No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA34630.1245892745292105No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA32520.11699807125873302No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG30780.1107380268555905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29970.1078238682541276No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT29570.1063847775867385No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA29450.10595305038652177No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT28690.10321877811848248No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT28240.10159980111766978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA20800.084.7798841
GCAAAAG97850.080.532843
GAGCAAA120250.067.7107541
CAAAAGC124050.064.105974
AGGCTCG3450.063.07149145
AGTAGAA33000.062.892951
AGCAAAA127200.062.8248672
AGCGAAA31450.060.2240141
CGAAAGC33400.057.3009383
GCGAAAG34000.056.0936852
AAGCAGG181450.051.934917
CAGTACG13800.049.901069
AGCAGGC28350.049.3488778
AAAAGCA163300.049.0085145
GCAGGGT43300.047.209179
AAAGCAG214450.044.991746
AGCAGGG83550.044.8556448
AGGTCGT2800.044.0120626
GGTCGTT2700.042.956537
GCAGGTA55000.042.306549
Produced by FastQC (version 0.11.5)