FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4875604
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA83790.1718556306049466No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC55000.11280653637990289No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG53560.1098530561546836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53050.10880703190825178No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT52860.10841733660075757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA25350.089.5795751
AGCGAAA49000.070.181861
GAGCAAA160150.063.920871
CGAAAGC55700.062.080933
GCGAAAG58050.059.5860822
GCAAAAG169650.059.3958323
CAAAAGC190800.053.9092674
AGCAAAA189550.053.4825442
AAGCAGG249800.047.299777
AAAAGCA234250.044.002715
AAAGCAG309400.039.6179126
AGCAGGG131950.038.8391238
CAGTACG8950.033.206289
GCAGGGT72950.030.9025619
AGTAGAA52400.030.8756561
AGGCTCG7300.030.803898145
TATCGTG6400.030.5923232
CGAGTCG1902.0698099E-730.542404145
TAGAAAC68850.029.4905432
ATATCGT6500.029.0203461
Produced by FastQC (version 0.11.5)