FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416570
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG152730.18146347027351997No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA123970.1472927807883734No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA109050.1295658445186103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96270.11438151170845132No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT91710.10896362770107063No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT89220.10600517788125091No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA38900.082.057271
AGCGAAA76700.072.829841
GAGCAAA228000.071.591531
GCGAAAG78550.071.26582
CGAAAGC83050.067.912043
AGCAAAA317550.050.5791632
CAAAAGC326650.049.1771054
AAGCAGG395700.047.610557
GCAAAAG349000.046.0726283
AAAGCAG461900.041.5561756
AAAAGCA409000.039.664535
AGCAGGG232300.038.2876138
GTAGAAA108250.035.1840131
TAGAAAC115700.031.39892
CAATTCG24600.030.6452929
GCAGGTA70650.029.7544149
CGTGTAG11550.028.8696425
GCAGGGT159600.027.8415349
TCGTGTA12000.027.787694
AGTAGAA102500.026.6828041
Produced by FastQC (version 0.11.5)