FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653335
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA19700.080.295331
AGCGAAA38850.079.5643541
GAGCAAA76900.076.051651
GCGAAAG44400.070.392112
CGAAAGC46600.067.3635943
CAAAAGC100900.057.1139144
AGCAAAA104250.055.508122
GCAAAAG105500.054.493413
AAAAGCA127800.045.6586235
AAAGCAG171600.042.199376
AAGCAGG180700.038.9109087
GCTATCG900.00363292332.2156648
GAAAGCA105500.030.9877514
TAGAAAC47450.030.2592872
GTAGAAA54700.027.4588891
AGTAGAA43700.027.0648821
TCGTCGG1100.0096449426.380144145
CCGACGC5050.025.857765145
AGCAGGC56250.025.257088
AGCAGGA82000.024.3090768
Produced by FastQC (version 0.11.5)