FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7803636
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA258480.33123021114772655No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG221900.28435462648437215No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC205970.2639410654212985No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT201720.2584948862299574No Hit
CATTTCACACCATCGATTTCTTTTACGATAACTTCTGGACGAGATACTTG149390.19143640221045677No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC146300.18747670957486995No Hit
CTCCAACAGACGCGGTTGAAGCTCTTCCAATCCTACACATCGATGAGCCA134790.172727174870791No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA133510.1710869138437518No Hit
GGTCAAAGGTATGGTTCATGATACCGTAACCACGAGTCATTGACAAGAAC128660.1648718622959861No Hit
CTATCACTCCGACAGATGCAGTTGAAGCTCTTCCAATCCTACACATCGAT126570.1621936235877737No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA122770.1573240986637511No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC120040.15382572944201908No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC120040.15382572944201908No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC118930.15240331558263354No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA113220.1450862136573259No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA108740.13934530006268872No Hit
GAGCAAAAGCAGGGAGCTTGAAAAGGAGATGGTATTGATTGCAAACAGGA95260.12207130112168227No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT94310.1208539198906766No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT92900.11904706985307874No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT88440.11333178533698907No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87860.11258854205911192No Hit
GGTCAATAACTCACCATTTGCTGGTAAAGAAGGTAAATGGGTAACTTCTC86740.11115331366045264No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC84930.10883388205190504No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG83160.1065657086004524No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT82810.10611719972587139No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG82730.10601468341168144No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT81770.10478448764140204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA46850.098.9580151
GCAAAAG254750.084.175283
GAGCAAA290950.074.960131
AGACGCG32750.073.924038
GACGCGG33450.071.9436349
AGCAAAA309700.069.560872
CAAAAGC311100.069.506594
GCGAAAG80600.063.5145452
CGAAAGC82900.062.0875243
GCAGGGT96300.061.5711759
AGCGAAA84150.060.52611
CGAGTCG4350.056.69847145
AAAAGCA387600.055.974485
AAGCAGG465650.054.6857957
TAGAAAC125900.052.3530122
AGCAGGG212200.052.2291038
CAGACGC48850.051.341387
AGGGTGT55450.050.329616
ACCATCG53400.048.866559
AGCAGGC45800.046.371668
Produced by FastQC (version 0.11.5)