FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53201
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1050.1973647111896393No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1010.1898460555252721No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT970.18232739986090488No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC930.17480874419653766No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC890.16729008853217045No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA680.1278171462942426No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG660.12405781846205899No Hit
TTCCCATTGAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTC600.11277983496550817No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT600.11277983496550817No Hit
GGTTTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT580.10902050713332456No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG560.10526117930114096No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT550.10338151538504915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA451.216853E-680.555561
CAAGGGT300.001925648272.58
GCAGGGT350.003547974662.1428579
ACAAGGG350.003547974662.1428577
ACAAGGT400.00601956654.3758
TCAGTAT400.00601956654.3758
TAGAAAC803.6868885E-545.31252
CAGTTTA657.273885E-444.6153879
CTATACA657.273885E-444.6153874
CACTGTG700.001047637341.428577
CCTATAC700.001047637341.428573
TACAGTT750.001470782938.6666687
GGACAAT750.001470782938.6666686
ATACAGT750.001470782938.6666686
AACACTG750.001470782938.6666685
TCCTGTT850.002718824234.117652
GGTGTGG900.003597673732.222221
TTACTCT900.003597673732.222224
CAACACT1352.1045626E-532.222224
GTAGAAA1352.1045626E-532.222221
Produced by FastQC (version 0.11.5)