FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7215116
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78800.10921515329760463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA221350.082.401651
GCGAAAG97050.068.071482
AGCGAAA100450.064.7154851
GAGCGAA60850.060.450171
AGCAAAA299450.059.839892
GCAAAAG301400.059.4366653
CAAAAGC307200.057.978874
CGAAAGC117900.056.5101743
AAAAGCA358900.049.7269025
AAAGCAG477500.044.7842646
AAGCAGG446650.044.2249077
AGCAGGG231750.034.1250088
ATACGGG16950.033.7895433
TACGGGC17400.032.079484
CGATTAC33550.028.761351
AGCAGGC140200.028.074998
GAAAGCA256450.026.7125244
ACGGGCT21850.025.5455995
AGTAGAA95700.025.4728491
TATACGG25050.023.1592562
Produced by FastQC (version 0.11.5)