FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10027617
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110140.109836664084797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA273750.076.850161
GCGAAAG111000.072.1211852
AGCGAAA121650.065.715881
CGAAAGC137850.058.2680663
GCAAAAG380750.054.757873
AGCAAAA388600.053.349222
CAAAAGC391950.053.1714864
GAGCGAA73600.048.099761
AAAAGCA469850.044.6176075
AAGCAGG551850.041.2316787
AAAGCAG611550.039.6015136
AGCAGGG279050.031.8722328
AGCAGGC177750.026.6696978
GAAAGCA314950.026.6065034
AGTAGAA126200.022.5909291
AACAAGG218300.021.5247442
GTAGAAA166350.021.4121151
AGCAGGT202250.019.783388
GCTAGCC29850.019.6715353
TAGAAAC170800.019.6142122
Produced by FastQC (version 0.11.5)