FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9802733
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG434240.44297850405596073No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA309660.31589149678972184No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA277900.2834923689138529No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA214370.2186839119253784No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT209610.2138281232386927No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT206230.21038010522167644No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT194180.1980876149539113No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAACGGTGAAATTTCTCCAACT185170.1888963006541135No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG176730.18028645684830955No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC167160.1705238732912546No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC164260.16756551463760158No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG163730.16702484909055465No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAGAAAAGAATAGAGA155800.15893526835832414No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA154930.15804776076222823No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT154290.15739488161107723No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC153320.15640536164761398No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC138760.1415523609589285No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCGT128370.13095327598946133No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT127710.13027999436483684No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT125020.12753586168265524No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC122840.1253119920740471No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG119630.12203739508155531No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA117260.11961970197494924No Hit
GCAGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT114850.1171612039213962No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT111060.11329493519817381No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT110720.11294809314912485No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG109290.11148931629577181No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG109260.11145871258556161No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC107550.10971430110357999No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT106910.10906142195242897No Hit
GATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCA106090.10822492054001676No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105950.10808210322570246No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC104550.10665393008255962No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT104190.10628668556003718No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT101190.10322631453901683No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC101010.1030426922777556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA34800.0115.513651
GCAGGTA90250.073.087049
GCAGGGT164350.072.903669
GCGAAAG80200.068.082112
CGAAAGC80500.067.9891053
GTAGAAA113800.065.802161
GAGCAAA318150.064.4301761
AAGCAGG394300.061.786647
AGGCTCG10650.059.257565145
AGCAGGG245450.058.9147228
AGTAGAA87450.055.8416251
TAGAAAC139800.053.372872
GCAAAAG419500.048.8174133
CAAAAGC437350.047.352494
GTGTTTT119650.046.7076349
AGCAAAA444400.046.013732
AGCAGGT189100.043.8892258
CAGGTAC27250.042.829963
AAAGCAG576800.042.7406236
GACAGTA23200.039.6797147
Produced by FastQC (version 0.11.5)