FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7816676
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAAGGTATGGTTCATGATACCGTAACCACGAGTCATTGACAAGAAC86340.11045615809072808No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83350.10663100274336561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA38550.093.1643751
GCGAAAG102350.075.452262
AGCGAAA103500.074.5882341
CGAAAGC107450.071.784223
GAGCAAA221600.068.4625851
CAAAAGC278900.053.5671164
AGCAAAA288550.051.6417852
GCAAAAG290800.051.1536643
AAAAGCA349500.042.9325265
AAGCAGG429600.041.0012327
AAAGCAG451850.040.907826
GAAAGCA255800.031.3417594
AGCAGGG264250.027.2105738
ACCGTCG18900.025.3116449
AGCAGGC156750.025.2017088
TACGTTC57300.025.067287145
TTTACGC34300.024.726384
TACGCCA34600.024.721026
TTACGCC35200.024.2997935
AGCAGGT144450.022.7813428
Produced by FastQC (version 0.11.5)