FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7348140
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG132890.18084848682795918No Hit
GTATTTAAATGGTCTGTTTGGTAGCGAACAAGGCAAGAGATTCGTTAAAT105650.14377788120531182No Hit
GTATTGGATGTGTTCAAAAGATACCAAGAAGAATATGACAGGTATTTAAA89410.12167705024672912No Hit
TACTAGGGACGGCCCTTCTCAAATTTCCTGCGCGCACAGAGGATAGGGAC87460.1190233174653722No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC86270.11740386002444157No Hit
GAGCAAAAGCAGGCAGCGGAAGGTATTGGATGTGTTCAAAAGATACCAAG85960.1169819845566361No Hit
TCGTAAAGGTGGTGCAGGCGGTTGGGAACAGCTTTGATGACCGGCCTTAA80120.1090343950986236No Hit
GAGCAAAAGCAGGGATAACAAGCAAGTCCCCTCCGTCATCCCTGCTGATT79070.10760546206250833No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77760.10582269798887882No Hit
TTGTAGTAGTGGGTGGCAACCTGGTATTCGTGTGGCTCCCATTCATGATA74820.10182168548775608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA243350.074.243481
GCAAAAG255650.070.068693
AGCAAAA261800.068.361952
GCGAAAG114000.060.2398762
CAAAAGC307100.058.6094134
AAGCAGG379700.054.674547
AGCAGGC152850.047.706378
CGAAAGC149200.046.4500433
AAAGCAG476850.045.8619086
AAAAGCA432000.042.3183635
GCAGGCT76450.041.4331329
GCAGGGA99150.036.918379
AGCAGGG159700.036.8548778
GAAAGCA196850.035.756444
AGCGAAA202900.034.0801241
GAGCGAA143750.027.1054571
AGCAGGT153900.025.9040648
AGCAGGA158100.025.2159128
GTTAGGT17750.024.9100919
GCAGGTG124750.022.9509379
Produced by FastQC (version 0.11.5)