FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5394292
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA190710.35354037193388865No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG163220.302579096570968No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC141110.2615913265355305No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT140290.26007120118821897No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC132880.24633445872044007No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA105950.19641131774104925No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC105500.19557710261142702No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT99520.18449131044444758No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT97020.17985678194654645No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA91240.16914175205939908No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC86880.16105913435905955No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA86360.1600951524314961No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC82510.1529579785447284No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT81120.15038118069989537No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA77810.1442450649686743No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC77610.1438743026888422No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG74020.1372191197658562No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT73400.13606975669837673No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG72990.13530969402472096No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG70920.1314723044284588No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA67980.12602209891492713No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT64830.12018259300757171No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA64370.1193298397639579No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG63200.11716088042694017No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG62770.11636374152530118No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG62750.11632666529731799No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG62610.11606713170143551No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA60680.11248927570105585No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT59010.10939341066445792No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG57080.10581555466407824No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA56460.10466619159659876No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTGCAGTTTGTTGGCTT56210.10420273874680866No Hit
GGTTTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT56080.1039617432649178No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA55950.10372074778302695No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT54770.10153325033201763No Hit
GGTGTGGAGACCATGGTCATTTTAAGTGCCTCATCAGATTCTTCTTTCAG54490.10101418314025269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCG3800.083.98708145
GCAAAAG129000.083.1143953
GCAGGGT59750.079.829179
GCAGGGG22150.076.580079
AGTAGAA24300.076.1317751
GAGCGAA28900.074.055311
AGCAGGG102650.063.0617688
GCGAAAG36950.062.801532
CGAAAGC37350.061.915283
CAAAAGC180950.060.852844
CGAGTCG2150.060.726387145
GAGCAAA196450.055.838251
TCACGCG553.2127614E-452.752216145
AGGGTGT36050.052.4832736
AAGCAGG245850.052.4545757
AGCGAAA45450.050.9200551
AGCAAAA215750.050.4842
AGCAGGC8100.049.22118
TAGAAAC57850.043.2464452
TAAACTA10250.043.1407435
Produced by FastQC (version 0.11.5)