FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8471308
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG87100.081.8434452
AGCGAAA87750.080.455441
CGAAAGC92150.077.8037953
GAGCAAA182300.059.4639321
GAGCGAA38750.053.7402731
GCAAAAG254550.042.1775933
CAAAAGC258400.041.79924
AGCAAAA286850.037.4158332
AAGCAGG388200.031.351747
AAAAGCA355250.031.0360725
AAAGCAG425950.030.1221716
GAAAGCA252550.029.7362754
AGCAGGG250850.023.5221397
AGCAGGC133600.020.1832078
TGACGCG8300.018.352377145
GCCGACC18950.016.459263145
TATACTG64500.015.1720995
GCAGGGG147400.014.9004328
GTATATA94700.014.7875781
TCGCTCC17950.014.5381695
Produced by FastQC (version 0.11.5)