FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5023155
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG284400.5661780295451763No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA190540.3793233535497113No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA157590.3137271296625328No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA135500.2697507841187461No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT129020.2568505252177168No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT118040.2349917531909726No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT117560.23403617845756303No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT117290.23349866767002014No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG112790.22454015454430534No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC104320.20767824206101546No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC90640.18044436215884238No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA86990.17317801262354038No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG85690.1705899977205561No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT84380.1679820750106258No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT80380.16001895223221263No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC79910.15908328530574906No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT76460.15221509190936772No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT76140.15157804208709466No Hit
ATTCTACACTGTAGAGACCCATTAGAACACATCCAGAAACTGATTGCCCC75790.1508812688439835No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG72360.14405289106149421No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA71510.14236072747108142No Hit
GATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCA69850.13905603151803997No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT65110.12961973102562035No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC64930.12926139050059177No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC62710.12484185735857245No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT61800.12303024692648347No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG60820.12107928184577221No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT60560.12056167886517538No Hit
GCAGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT60440.12032278518182297No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC60230.11990472123595629No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG58900.1172569829121339No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC58390.11624168475788622No Hit
GTAGAGACCCATTAGAACACATCCAGAAACTGATTGCCCCCAGGGAGACT58020.11550509590088301No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC57480.11443007432579723No Hit
GAAAATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACC57210.11389256353825435No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG56580.11263837170065429No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA56360.11220039994784155No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC56140.11176242819502882No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT54810.10911468987120646No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC54260.10801976048917462No Hit
GTAGGTACAGATGCAATTGTCATTCTGAGTGTCTCGCTGGATTCCTCTTT54190.10788040584055239No Hit
GAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAG51970.10346087269853309No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA51910.10334142585685689No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC51670.1028636384901521No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG50880.1012909217414155No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC50690.10091267340944086No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA50570.10067377972608849No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC50270.10007654551770749No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT50250.10003672990381543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA20350.0111.215031
GCAGGTA44400.075.917279
GCAGGGT99250.074.78949
GAGCAAA157900.071.528611
CGAAAGC47650.071.201333
GCGAAAG48000.070.691312
GTAGAAA59550.064.804211
AAGCAGG209600.064.568947
AGCAGGG136800.062.1559228
AGGCTCG3950.053.250328145
AGTAGAA48500.052.946211
AGCAAAA212950.052.943682
TAGAAAC74650.051.6705632
GTGTTTT66600.049.523099
GCAAAAG233700.048.36073
TTCGCTC8700.048.327764
GCTTTCG8600.048.0783651
CGGCGGC2900.047.52006145
CAAAAGC242100.046.9803284
AGCAGGT88750.045.8210538
Produced by FastQC (version 0.11.5)