FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8670085
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA129030.1488220703718591No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA119580.13792252325092544No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT112600.12987185246742103No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA97610.11258251793379188No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG89950.10374754111407213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG104750.076.360062
CGAAAGC111350.071.941853
AGCGAAA113950.069.918331
GAGCGAA36550.067.8956151
GAGCAAA199800.061.8111881
AGCAAAA297350.041.337732
TAGAAAC116850.039.842922
CAAAAGC313600.039.389054
GCAAAAG316300.039.009463
GTAGAAA130650.036.4887431
GAAAGCA232200.035.402414
AAGCAGG409750.033.983487
AAAAGCA408700.030.4180565
AGTAGAA120050.029.3749491
AAAGCAG511650.028.8737496
AGCAGGG214500.028.1837147
CTACACT63450.025.4774064
GGGTGTT104050.024.8705627
CAGTAGG80450.024.6871769
ACAAGGG155100.024.3520453
Produced by FastQC (version 0.11.5)