FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6882608
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG251710.36571892515162857No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA165090.2398654696010582No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA133400.1938218768234367No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA123640.17964120577548512No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG116880.16981934754964978No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT105230.15289262442376494No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT94610.13746242703347336No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC93400.13570437252855314No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC85880.124778281721115No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA85820.12469110546467269No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC84850.12328175598552177No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT84560.12286040407938387No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC83440.12123311395912711No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT81920.11902464879592153No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT80230.1165691842394627No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG79220.11510171725601691No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT78840.11454960096521552No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT77210.112181312665199No Hit
GTACTATGTTCTTTTCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG77160.11210866578483039No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT71990.1045969783547167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA32750.0108.123731
GAGCAAA231850.068.2591861
GCAGGTA62300.067.2484059
GCGAAAG64000.065.7172852
AGGCTCG7200.065.48643145
CGAAAGC66450.063.3845333
AAGCAGG326300.058.268377
GCAGGGT130750.056.4904069
GTAGAAA75750.055.4636571
GCAAAAG295650.053.2088623
AGCAGGG197800.052.402878
AGCAAAA305700.051.6172642
CAAAAGC322900.049.140424
TAGAAAC89750.046.539272
AGTAGAA57100.045.7487221
AAAGCAG429950.045.0147066
AGCGAAA97350.043.0828481
AGCAGGT138350.040.9706578
AAAAGCA399450.040.0495725
CAATTCG13000.039.029859
Produced by FastQC (version 0.11.5)