FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075612
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATAACACTGAAATTCGCTT29530.1422712915516002No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC29410.1416931488158673No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG28460.13711618549131532No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC26210.12627600919632379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA12700.090.254531
GAGCAAA67750.075.3839651
AGCGAAA22250.065.536261
GCAAAAG77450.064.768733
AGCAAAA78900.063.138832
CAAAAGC81000.061.9271164
CGAAAGC24450.059.8898473
GCGAAAG25700.057.8412252
AAAAGCA103900.048.485085
CGAGTCG604.931335E-448.36308145
AAGCAGG128050.045.3410727
AAAGCAG140150.042.8746226
AGCAGGG66450.040.031288
AGTAGAA31350.039.107931
TAGAAAC37700.037.1221162
CTTAGCG4150.036.6933982
CATACCG2252.0008883E-1135.43559
GTGCGTC1751.0121221E-733.1352926
CGCGGAT1102.4406613E-432.9462288
CAGTATG20100.032.8150989
Produced by FastQC (version 0.11.5)