FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292913
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG50050.080.120572
CGAAAGC50650.079.2933653
GAGCGAA20900.073.252481
AGCGAAA54350.073.02541
GAGCAAA102350.064.4409941
CAAAAGC144450.045.6176264
AGCAAAA149850.043.503672
GCAAAAG156000.042.1491933
AAAAGCA184350.035.743495
AAGCAGG210600.035.69377
GAAAGCA115650.035.1644254
AAAGCAG237600.033.6515666
AGCAGGG138350.026.249728
TATACCG2301.1059037E-625.2138045
TACGACG1500.001496413624.1629497
GCTTTCG12400.023.9911
ACAAGGG56950.023.4223613
AGGGTGT52900.022.3361426
GGGTGTT48100.022.002857
CTTTCGC13950.021.3124122
Produced by FastQC (version 0.11.5)