FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7685845
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC92000.090.225953
AGCGAAA95100.087.11681
GCGAAAG109900.075.806572
GAAAGCA208700.040.6748244
AAAGCAG314650.035.4791265
AAGCAGG288350.035.044736
AGGTGCG27850.032.0148437
GGTGCGT27650.031.7220828
GTGCGTT28950.029.5464219
AGCAGGG194400.028.0409727
GAGCGAA30050.027.5217881
TACGTGC11900.024.3660147
CGCCGTT6900.023.126541145
GCAGGGC64000.021.7466668
TAAGGTG63050.020.1202285
TGACGCG12000.019.946644145
GAGCAAA148200.019.0422251
CGTAGAC21100.017.8663043
GCAAAAG157950.017.5330543
AGCAGGC94950.016.6430537
Produced by FastQC (version 0.11.5)