FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4890908
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG258490.5285112702999116No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA156560.3201041606180284No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA136040.27814876092537416No Hit
CATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC124510.2545744062247746No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA91410.18689781120397275No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT89890.18379000381933172No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC89600.18319706688410414No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT88630.181213795066274No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC83250.17021379261274186No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC82350.1683736435034149No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT78880.16127884638189882No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA74420.1521598852401231No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG73640.15056508934537308No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT73110.14948144598099167No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA70910.14498330371374804No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT66010.1349647141185236No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT65190.1332881338189146No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC65170.13324724161648513No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT63850.13054835625613895No Hit
ACTATGATGAGAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAA58750.12012084463661962No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC58510.11963013820746578No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG57520.11760597418720613No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT55120.11269890989566764No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG52650.10764872289562592No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG51160.10460225381462909No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA50540.103334595539315No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG50070.10237362878222205No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAA49100.10039035696439189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA28150.0108.775911
GAGCAAA155750.086.839341
GCAGGTA47900.069.31039
GCGAAAG60750.067.446732
CGAAAGC62850.065.401853
AGCAAAA219800.060.7744032
GCAGGGT111800.060.104339
AAGCAGG270550.059.6429027
CAAAAGC244650.054.786964
AGCGAAA74300.054.1027831
AGGCTCG7400.053.909492145
AGCAGGG182250.053.73488
GCAAAAG257200.052.201413
GTAGAAA68800.051.678071
AGTAGAA49050.049.556941
TAGAAAC73150.048.2806782
AAAAGCA287550.046.738795
AGCAGGT94050.044.857188
AAAGCAG377150.043.5538836
ACAAGGG91300.041.4487953
Produced by FastQC (version 0.11.5)