FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6316894
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA37500.072.9479451
GAGCAAA183600.071.059141
AGCGAAA68300.064.4868241
CGAAAGC70600.062.9437873
GCGAAAG75050.059.0361822
GCAAAAG225500.057.0622523
AGCAAAA227500.056.3226282
CAAAAGC233100.055.292034
AAAAGCA279650.046.4507525
AAGCAGG324400.046.3436937
AAAGCAG360750.043.0202646
GCTTTCG23350.039.1549451
AGCAGGG191000.035.7121128
TCGCTCC24200.035.645215
CTTTCGC29850.030.1234762
CGCTCCC28900.029.5969456
TCCCGTC47000.028.080272
CTCCCGT52900.024.964261
AGCAGGA138350.024.7822828
CCGTCTG52250.024.1399294
Produced by FastQC (version 0.11.5)