FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6924121
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG148470.21442432909534653No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA102600.14817765316348458No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA92570.1336920599741108No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA75370.10885136178296134No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT74840.10808592166428056No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT73340.10591958170575008No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG72280.10438870146838855No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC70240.1014424791247871No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT69350.10015711741605901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA37950.080.482611
GAGCAAA179050.071.921011
GCGAAAG68000.065.154972
AGCGAAA68750.063.6321141
CGAAAGC69850.063.516613
CAAAAGC256850.049.361754
AGCAAAA258300.049.0156822
AAGCAGG309600.048.5596547
GCAAAAG271950.046.5433353
AAAAGCA311700.040.9775775
GCGAAGC9000.039.4689753
AAAGCAG391450.039.462486
GTAGAAA104850.039.2325331
AGCAGGG199900.038.800358
TAGAAAC105700.038.554632
GCAGGGT104100.036.139439
TCGCTCC25200.033.6560025
GCAGGTA51900.033.1013459
GCTTTCG26750.032.545371
CTTTCGC27750.031.879542
Produced by FastQC (version 0.11.5)