FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971976
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10830.11142250425936442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA20000.068.555361
GAGCGAA6250.053.3933831
GCAAAAG25950.051.399023
CGAAAGC8200.051.2729763
CTTTCGC3700.050.954212
CAAAAGC26150.050.174294
GCGAAAG8350.049.4990432
AGCAAAA29150.046.019472
TCGCTCC4050.044.7465255
AAGCAGG38350.041.5823827
GCTTTCG4550.041.454491
AAAGCAG39100.041.340926
GCAGTCG1252.7230635E-740.591966
AGCGAAA9700.040.3860741
AAAAGCA34700.038.438175
CGCTCCC5250.034.5169756
AGTAGAA16950.034.2396971
GCGAAGC850.002743918234.1126483
TTCGCTC5400.033.5598954
TAGAAAC17750.032.262883
Produced by FastQC (version 0.11.5)