FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5659623
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT92490.16342077908722893No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA84960.15011600596011432No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT77240.13647552142607378No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC74190.13108646989384273No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC69530.12285270591345042No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG69090.12207526897109577No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA65470.11567908321808715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63920.11294038489842875No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG58240.10290438073348702No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT57530.10164988021286932No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC57360.10134950684877773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC27150.059.8118483
AGTAGAA36250.058.251711
AGCGAAA33850.047.1615071
GCAAAAG113600.046.58583
CGCCGTT2800.044.040993145
AAGCAGG154050.039.8081177
CAGTACG16000.038.9609839
GCGAAAG41300.038.8061832
AGGCTCG4700.038.584103145
GAGCGAA23150.037.3009261
CAAAAGC152700.036.5997664
GAGCAAA157500.035.3377151
TAGAAAC72000.034.6485182
AGCAGGT78150.033.390668
CGACTCG1551.4605994E-632.759144145
CCGTATA5350.032.54046145
TGACGCG5400.032.23916145
GTAGAAA82350.031.3696781
AGGGTGT28750.031.0123086
GCTCGGA2651.382432E-1030.0894076
Produced by FastQC (version 0.11.5)