FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5471332
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC204890.37447919446306677No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT185570.33916786625267853No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA180180.32931651744036006No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC152720.27912764204402146No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT140390.25659199624515566No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT135600.2478372725325387No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT131200.2397953551347277No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG124990.2284452853528172No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT119610.21861221362549374No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA116670.21323875063695644No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC112850.20625690416885686No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA112040.20477646028425983No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC108010.19741079503126477No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA101860.18617038775932443No Hit
GGAGTGGAGGAAACACTAATCAACAGAGGGCCTCCGCAGGCCAAACCAGT95980.17542346178224974No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT94680.1730474407328965No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA91980.16811262778423974No Hit
ATGCCCAAGCAGAAAATGGAAGGACCTCTTTGCATCAGAATGGACCAGGC89970.1644389337002397No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG87530.15997932496145362No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA85820.15685394342730435No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT85060.15546488496768246No Hit
CTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGT84480.15440481403797102No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT82610.15098699914390135No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAA81840.1495796635992844No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG80450.14703914878497595No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA74550.1362556686379112No Hit
CTTGAATGGAATGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATT72720.13291096208382164No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT71320.13035217018451814No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT70210.12832341375007036No Hit
GTAATGAGAATGGGGGATCTCCACTTACTCCAAAACAGAAACGGGAAATG70200.12830513666507534No Hit
GTTTTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAA69820.1276106074352644No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA68600.12538080306587135No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG66100.1208115318171151No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT65540.11978801505739371No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC65160.11909348582758277No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA64360.11763131902798075No Hit
TTTTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC63980.11693678979816981No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC62370.11399417911397078No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC60770.11106984551476679No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC59380.10852933070045832No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTGTCGTTCCATCAGGCCCC59320.10841966819048816No Hit
GGTTTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT58570.1070488868158613No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC58260.10648229718101551No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57890.1058060450361996No Hit
ACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGCTA56590.10343002398684635No Hit
TCTTATCTCTTCAAACTTTTGACCTAGCTGTTCTCGCCATTTCCCGTTTC55860.10209579678220954No Hit
GGTGTGGAGACCATGGTCATTTTAAGTGCCTCATCAGATTCTTCTTTCAG55860.10209579678220954No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG55470.10138299046740355No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT55040.10059707581261748No Hit
GATGCAACGATTCAAGTGACCCGCTTGTTGTTGCCGCGAATATCATTGGG54930.10039602787767221No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA18500.079.605921
CGAAAGC40100.079.183213
AGCGAAA42400.074.9426961
GCAAAAG94650.070.7709053
AGTAGAA44200.065.48951
GCGAAAG51500.061.95382
GCAGGGT63550.055.5475859
AGGGTGT66350.053.0951736
TAGAAAC77850.051.043972
CGAGTCG4200.048.358204145
CAAAAGC144700.047.6909644
ACAAGGG86800.046.7704623
CAAGGGT86200.046.6716734
AAGGGTG80750.044.3449445
AGCAGGG98650.043.8660968
GAGCAAA164100.042.087141
AGCAAAA167350.041.0776022
TCATACT38750.039.672232
AGGCTCG6900.038.89682145
GGTGTTT90150.038.7554788
Produced by FastQC (version 0.11.5)