FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5236436
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC144990.27688679857826964No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC140930.2691334335032453No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT139960.26728102854689717No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG132000.2520798497298544No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA122320.2335939940829984No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA100190.1913324253366221No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA90850.1734958662724036No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT89350.17063132252547342No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA87290.16669734911302267No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC87030.1662008281968881No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC84480.16133110382710683No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT82250.15707248212333733No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT74680.14261608468049644No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC73400.14017167401644937No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA71860.13723074243626773No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT64970.12407293815870184No Hit
GAGCAAAAGCAGGGAGCTTGAAAAGGAGATGGTATTGATTGCAAACAGGA62670.11968063774674224No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC62250.1188785654976018No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA56360.10763045705132268No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT54830.10470862242945393No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT54660.10438397413813516No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA54300.10369648363887193No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG54260.10362009580562045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA27100.080.314321
GCAAAAG143100.080.197873
GCGAAAG49750.067.338232
GAGCAAA175950.066.015071
AGCGAAA50900.065.423831
CGAAAGC51500.065.177143
CAAAAGC183200.063.819854
AGCAAAA182500.063.0957952
AGTAGAA57750.061.1812021
GACGCGG10450.056.8774729
GCAGGGT61450.056.3830769
AGACGCG10750.055.9644668
AGGGTGT38050.052.3866656
TAGAAAC91100.051.9765662
CGAGTCG3700.050.97322145
AAAAGCA240950.048.7944955
AGCAGGG142000.047.8292968
AAGCAGG295950.045.8245477
ACAAGGG81000.041.97683
AGGCTCG6700.040.05875145
Produced by FastQC (version 0.11.5)