FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4639639
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA109200.069.371821
AGCGAAA52550.066.554821
GCGAAAG53100.065.8228452
GCAAAAG143200.052.24323
GAGCGAA30150.050.7808951
CGAAAGC70150.050.429573
AGCAAAA149650.049.7158472
CAAAAGC161500.047.0395434
AAAAGCA200700.038.067435
AAGCAGG234400.035.9037327
AAAGCAG256050.035.189366
AGTAGAA71600.031.517561
GCCGACC11700.026.658632145
GTAGAAA91650.026.5226881
GAAAGCA139050.026.1702024
AGCAGGG140700.024.7287868
TAGAAAC96550.024.6271783
AGCAGGC72050.022.6362348
TCGCTCC15450.021.5819025
TGACGCG6450.021.367277145
Produced by FastQC (version 0.11.5)