FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4912697
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA123620.25163367494474015No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT102040.2077066833146844No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT93310.1899364035681419No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC86170.17540263525310026No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC85320.17367242473940483No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT79200.16121490904079774No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG76750.15622783167779328No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA76200.15510828369834329No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC75370.15341878402026424No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAGAAAAGACG69720.14191797295864167No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC68480.13939390115042716No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT64170.1306207160751009No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC63210.12866659596551547No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG62370.12695674086962822No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG60870.12390342819840101No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG59140.1203819409175856No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG58020.11810213412306926No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA55850.11368500845869386No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG55750.11348145428061206No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA55450.1128707917463666No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC54220.11036707535596027No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA54190.11030600910253573No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53190.10827046732171759No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA52740.10735447352034941No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG51880.1056039075888458No Hit
CTATTGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA50920.10364978747926037No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT50860.10352765497241129No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT50280.10234704073953675No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG50040.1018585107121404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA29850.078.7484741
GAGCGAA22000.065.955161
GCAAAAG122800.065.407723
CGAAAGC33900.065.007223
AGGCTCG4650.062.39774145
AGCGAAA36750.059.0276261
GCAGGGT45050.058.8889969
GCTCGGA2500.055.0919274
CAGTACG16800.053.932269
CAAAAGC162750.052.1567274
AGGGTGT29800.051.082086
AAGCAGG190600.050.0473257
GCGAAAG45800.048.1274722
GAGCAAA180400.046.0077481
AGCAGGG83850.044.6925778
GCAGGGG21400.042.3393449
AGCAAAA195750.041.8563772
TAGAAAC60650.041.7233852
TATATTC25250.041.3417433
CAAGCTA17550.041.3044934
Produced by FastQC (version 0.11.5)