FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6871948
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCACACCATCGATTTCTTTTACGATAACTTCTGGACGAGATACTTG157710.22949824416599193No Hit
CTCCAACAGACGCGGTTGAAGCTCTTCCAATCCTACACATCGATGAGCCA138030.20086007635680597No Hit
GAGCAAAAGCAGGAGAAGCGAATGTGTATCGTTTGGCAGAAGTTTCTGTA134800.19615980796129423No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG121630.17699493651581763No Hit
AAACAAGGATTTGCAAAAAAGGCTCCTTTTTTGTGGATTTTCCAAGCGGC115350.16785633418646356No Hit
GAGCAAAAGCAGGTGTGTAGTTAAAACTAATACTCGGTGTCATCATATGT109900.1599255407636961No Hit
GAAATATACATTCCCATACCCACCAGAGGCAATTACTACAGCATGTGCAG92590.13473617669982368No Hit
GTGTAGCACAAGGGGTTCCTTTTGGTCGTGAATATGGCGGTCAGTTGGAT90080.1310836461509895No Hit
GTTGAATATCCAATCAAACCACGCGCTGGAACAAGGAAGACCAAACGAGT84700.12325471612998236No Hit
TTCCCATACCCACCAGAGGCAATTACTACAGCATGTGCAGAATGTCTCTC81530.11864175922169376No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74170.10793155012232339No Hit
ATATAATGGTACAAACGGGACTTAATTTCCGTAACGATGTGAATACCACA73590.10708753907916649No Hit
CATTTCACACCATCGATTTCTTTTACAATAACCTCTGGACGAGATACTTG72270.10516668636025768No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA71830.1045264021206214No Hit
ATTCTAAAGTGAATAATTATAGCATACAATGGATACACACTCAAGATGCC71240.10366783916292731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA42100.098.057221
GAGCAAA205300.090.999221
CAAAAGC281850.065.505984
AGCAAAA302000.061.131762
GCGAAAG99000.060.9401552
GACGCGG50750.060.5597469
AGACGCG51400.060.216988
GCAAAAG307150.059.9489633
AAGCAGG370550.059.5663577
CGAAAGC101750.059.3484043
AAAAGCA333050.055.8262525
AGCGAAA108950.055.138191
AAAGCAG424700.053.115476
TCACACC84800.050.262265
TTCACAC86500.049.8621374
AGCAGGT131950.048.6719328
ACCATCG86650.047.849979
AGCAGGG207450.046.2275288
TAGAAAC91950.045.3452222
GCAGGGT74500.043.88089
Produced by FastQC (version 0.11.5)