FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6593422
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69900.10601475227886219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA87200.076.973431
GCGAAAG88650.076.078632
CGAAAGC90700.074.495283
GAGCGAA36400.067.579331
GAGCAAA134800.064.004061
CAAAAGC197300.043.3539734
GCAAAAG199300.043.0325973
AGCAAAA208600.040.883822
AAAAGCA261350.033.311275
AAAGCAG338550.032.609286
AAGCAGG324700.032.3697937
GAAAGCA229350.030.436984
AGCAGGG213500.023.7317758
TACCCCG25150.022.7698295
CCGCTCA29250.020.3207119
TAGCGAC9000.018.5250474
GTATAGG32400.017.4688851
AGCAGGC111650.016.3603558
CCCGCTC38150.015.7701028
GGAGCGA19650.015.509661
Produced by FastQC (version 0.11.5)