FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4745110
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTAAATGGTCTGTTTGGTAGCGAACAAGGCAAGAGATTCGTTAAAT67290.14180914667942365No Hit
GTATTGGATGTGTTCAAAAGATACCAAGAAGAATATGACAGGTATTTAAA60330.12714141505676368No Hit
TCGTAAAGGTGGTGCAGGCGGTTGGGAACAGCTTTGATGACCGGCCTTAA47610.10033487105672997No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG78700.061.1828232
GAGCAAA96150.050.857781
CGAAAGC102200.048.021093
GCAAAAG119600.040.3680083
GAAAGCA130650.037.6731344
AGCGAAA132550.036.6178781
AGCAAAA137050.035.7687532
AAGCAGG180200.035.760966
CAAAAGC145150.033.859774
AGCAGGG92500.032.0508277
AAAGCAG220650.031.8336645
AGCAGGC58100.025.9496278
ATGTGTT41600.025.2649578
AAAAGCA220550.022.9740165
TGGATGT47050.022.955855
GGATGTG50700.021.3029886
GCAGGGG44850.018.747338
ATTGGAT59950.018.7428993
TAGGCCG4303.7585778E-818.5426889
GCAGGGC45650.017.783668
Produced by FastQC (version 0.11.5)