FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6180781
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA269390.43585106801227874No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG246120.3982021042324586No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT237480.3842232882867068No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC198100.3205096572747036No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC148360.2400343904758962No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA129650.20976313511188957No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA125840.20359886558025597No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC125660.2033076402480528No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA120170.19442526761585632No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC111330.18012286796765653No Hit
GAATCACAGTCTCTACCAAAAGAAGCCAACAAACTGCAATCCCGAATATC111000.1795889548586174No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC109000.1763531178341378No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA105570.1708036573371553No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT103400.16729277416559493No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT102110.16520565928480557No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT99550.16106378789347173No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT90950.1471496886882095No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC85550.13841292872211458No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG83780.13554921295545014No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG83210.13462699940347345No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG81340.13160149178558503No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT79910.12928786831308212No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG78660.12726547017278236No Hit
ACTATGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCT76620.1239649164078132No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA76390.12359279514999803No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTGCAGTTTGTTGGCTT76170.12323685307730528No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC75540.12221756441459422No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT75390.12197487663775823No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT73790.11938620701817455No Hit
GGTCACCTCTAACTGCTTTTATCATGCAATCTTCTTGTGAAAACACCATG71330.11540612747806467No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT70340.11380438815094726No Hit
CAAGTTGTAGACCAAGAACTGAGTGATGCCCCATTCCTTGATCGGCTTCG69600.11260712845188982No Hit
CATCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCA68240.1104067592752437No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG67910.10987284616620456No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC67800.10969487512985819No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT65640.10620017114342023No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA65610.10615163358805305No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT65190.10547210781291232No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT64730.10472786529728201No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64680.10464696937167002No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT62750.10152438664304722No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT62520.10115226538523206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA29850.097.210491
AGGCTCG7450.088.59994145
GCAAAAG152350.087.1743553
GCAGGGT77050.077.049149
AGTAGAA30750.076.6718141
GCAGGGG28450.069.556379
CAAAAGC204300.066.246684
CGAAAGC50200.065.996093
GCGAAAG51500.064.346832
AGGGTGT48000.061.9184766
AGCAGGG129700.061.58838
AGCGAAA54450.061.5519181
CGAGTCG3100.056.15621145
ACCTCTA24350.055.6698765
AAGCAGG308550.052.8357057
GAGCAAA255500.052.725231
CAAGCTA14500.052.4939734
GGTCACC26950.051.41291
AGCAAAA263400.051.2604142
GCTTATA8400.050.068772
Produced by FastQC (version 0.11.5)