FastQCFastQC Report
Mon 29 Oct 2018
HMLHWBGX7_n02_HK_S01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK_S01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4403066
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49990.11353452344343691No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA130050.091.766061
GAGCGAA26950.078.8742751
GCGAAAG55300.069.75832
AGCGAAA55150.069.325281
CAAAAGC178150.066.406494
AGCAAAA176550.066.043142
CGAAAGC60800.063.789053
GCAAAAG192700.061.0942343
AAAAGCA221100.054.0312045
AAAGCAG269050.051.703296
AAGCAGG282050.048.62527
AGCAGGG161500.035.458148
AGCAGGC88700.032.4435428
TATCGTG12850.031.6005252
AGTAGAA72450.029.5399781
TAGAAAC77500.029.0047682
TCGCTCC15850.028.3555835
ATATCGT13150.028.1365571
TCGTGTA13500.027.9219514
AGCAGGA115650.027.8290738
Produced by FastQC (version 0.11.5)